rabbit mannose receptor 1  (Cell Signaling Technology Inc)

Journal: Materials Today Bio

Article Title: Hyperthermia/glutathione-triggered ferritin nanoparticles amplify the ferroptosis for synergistic tumor therapy

doi: 10.1016/j.mtbio.2024.101085

Figure Lengend Snippet: The mechanisms underlying occurrence of ferroptosis. (A) A schematic illustration of GSH-triggered and PTT-enhanced ferroptosis. (B) GSH contents normalized to the total protein concentration in MCF-7/ADR cells after different treatments ( n = 5). (C) Cellular protein expression of GPX-4 in MCF-7/ADR cells after treatments with I@P and I@P-ss-FRT (50 μg Fe/mL) with or without 808 nm laser exposure (1.25 W/cm 2 , 5 min) and (D) the relative level of GPX-4/β-actin ( n = 3). (E) CLSM images of MCF-7/ADR cells after incubation with I@P and I@P-ss-FRT showing intracellular Fe 2+ ions levels using fluorescent probe FerroOrange and (F) the quantification of fluorescence intensity inside cells; Scale bar = 50 μm ( n = 3). (G) CLSM images, (H) mean fluorescent intensity of the cells determined from the CLSM images, and (I) FCM analysis of ROS production in MCF-7/ADR cells after different treatments; Scale bar = 50 μm ( n = 3).

Article Snippet: Cell lysis buffer, phenylmethanesulfonylfluoride (PMSF), SDS-PAGE sample loading buffer, nonfat powdered milk, HRP-labeled goat anti-mouse IgG (H + L), HRP-labeled goat anti-rabbit IgG (H + L), BeyoECL Plus, BCA protein assay kit, Calcein/PI cell viability/cytotoxicity assay kit, reactive oxygen species (ROS) assay kit, mannose receptor C-type 1 (MRC1) rabbit polyclonal antibody and β-actin mouse monoclonal antibody were bought from Beyotime Biotechnology Co., Ltd (Shanghai, China).

Techniques: Protein Concentration, Expressing, Incubation, Fluorescence